After estimation the optical operating conditions of He-DBD operation, the spectroscopic characteristic of the He-DBD-based reaction-discharge system in relevance to the several optical temperatures in addition to electron number density has been carried out. Then, the resulted model was validated in order to confirm its accuracy. Based on the suggested statistical model, the optimal operating conditions under which the T rot(OH) is less than 37 ☌ (310 K) were estimated. To evaluate the effect of the He-DBD operating parameters on the rotational gas temperature T rot(OH), a design of experiment followed by a Response Surface Methodology was applied. To make this He-DBD-based reaction-discharge system safe for biological purposes, a multivariate optimization of the operating parameters was performed. In this work, we present the portable helium-dielectric barrier discharge (He-DBD)-based reaction-discharge system of controlled gas temperature for biological activities. For that reason, controlled promotion of these processes is required. Proliferation, migration, and angiogenesis are key factors in the wound healing processes. Skin injury leading to chronic wounds is of high interest due to the increasing number of patients suffering from this symptom.
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